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Hey Guys,
I have noticed that over the past couple of days, blocking my nitrocellulose membranes in 5% TBSTM for more than two nights is causing them to go green, sometimes associated with a ver raw smell! This has never happened before, we used to block for a week sometimes with no issue whatsoever. Anyways, I washed several times with PBS until I could no longer smell that pungent smell and tried adding an old (frozen, reused) antibody I had that has been working perfectly fine, only when I developed I did not see anything. Any ideas as to what could be the source of the this milk going bad, and could it have deteriorated my proteins?
Thanks
