Let me clear my work more. I am going to do immunoblotting after native gel electrophoresis and transferring. To avoid the poor recognition of antibody due to the possibility of epitope embed in the complex, I want to use SDS to break and loose the complex, and let the epitope available for antibody binding. This is just a quick and dirty job. Of course, 2D will be the next choice if above test is not workable. So, 0.05% is enough? thanks for the kindly reply.
I also have this problem, and I am going to soak the gel (after electrophoresis) in 2% SDS for 10min before transferring and the following immunoblotting. Is there any better suggestion available? thanks
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