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Wurmbox

Member Since 07 Dec 2012
Offline Last Active May 03 2013 12:30 AM
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Topics I've Started

Staining red blood cells for confocal microscopy

15 April 2013 - 06:45 AM

Hi there,
I plan to stain red blood cells and visualize them under the confocal microscope. For "normal" membrane staining in our lab we use DiD or DiO stains, however, they both seem not to work with RBC. I suppose that the RBC may undergo hemolysis during staining, but I am not sure. Also, the literature on that topic is really rare to my knowledge....
Does somebody have experience with staining red blood cells for confocal microscopy? I appreciate your valuable answers!
Best,
Wurmbox

THP-1 differentiated cells detach when washing with PBS (Mg and Ca free PBS)

07 December 2012 - 06:58 AM

Hello there,
i am relatively new to THP-1 cell culture. I have succeeded to differentiate my THP-1 to adherent cells. these differentiated cells will be used for a toxicological study. however, if i remove the medium and wash the cells with warm PBS, even the control cells (non treated) do detach to a certain extent. does anyone have an idea how to circumvent this problem? precoating the 96WP with poly-d-lysine?
thanks in advance for your help!
best,
wurmbox

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