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Hi all,
I have the following problem:
after transformation of XL-1 blue cells (form Quickchange kit, Agilent) with either pUC18 (control plasmid of the kit) or my pET plasmid+insert, the cells grow well on agar+Amp plates, but when I pick the colonies, inoculate them in freshly made LB+Amp medium and incubate them overnight @250rpm, 37°C, all I obtain the next morning is a cell lysate at the bottom of the culture tube (round bottom 14mL falcon). What could cause this unwanted lysis? Should I still attempt to purify DNA out of this lysate?
Thanks a lot for your help!
Cheers
A
