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Hi,
Thank you all for your answers. I isolated just a little bit of DNA from the lysed cells, enough to retransform into TG1 cells, which grow normally and express my DNA much better. I'll switch to this strain for the next mutagenesis.
Also tried to use minimal medium M9 with XL-1 blue cells, but obtained the same result: cell lysed already after overnight incubation. TG1 it is!
Good luck with your experiments!
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In Topic: unwanted cell lysis during LB+Amp incubation
07 December 2012 - 06:09 AM
prabhubct, on 07 December 2012 - 05:31 AM, said:
Cell lysate you are considering may be overgrowth. Try to isolate plasmid.
Could be, but the rest of the incubating solution is clear... not the cloudy suspension you're supposed to observe
