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I have been trying to amplify the HIV gene from HIV infected patients' genomic DNA. I am not getting the respective band, instead I'm getting a non-specific band with all the samples.
I checked the template DNA with other genes, its working fine. I checked the primers by using them with PNL4-3 as template, I got the exact band length. I tried changing the cycling conditions, template quantity, MgCl2 conc., tried adding DMSO (2%), everything possible, still no use.. What else can I try doing so that I get the required amplification..??
Suggestions would be greatly appreciated...
Thank you in advance..
