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Hi Paridhy,
Welcome to BioForum.
It would be great if you could post some information.
1. Gel picture of your unspecific bands that you are getting.
2. How are you extracting gDNA from patient samples ( Often blood proteins (contaminants) do not let your PCR work. Hence the non specific amplification. Am guessing here that your positive control template is purified with a protocol different from the one you are using for extracting gDNA.
#146329 HIV gene- PCR
Posted
on 06 December 2012 - 02:03 AM
