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How would you would quantitate the average number of cells with nuclear localised GFP signal?
How could you quantitate the average proportion of total GFP that is localised to the nucleus per cell
How could a cell maintain a cytoplasmically localised transcription factor such that after a stimulus this factor would relocate to the nucleus?
How could you use recombinant Ran protein to purify multiple nuclear import factors and iclude two control incubations to ensure the factors isolated are specific Ran binding partners?
