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Mehak-khushboo

Member Since 04 Dec 2012
Offline Last Active Dec 19 2012 04:02 AM

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RNAi, stem cell biology, vectors, enzymes

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Fluffy
04 Dec 2012 - 11:32

Topics I've Started

Inclusion body formation of the recombinant protein expressed in pET28a in E.col

04 December 2012 - 08:44 AM

Hello...
I am new to the forum and really looking forward to get help through this..........I have expressed a protein of nearly 60kd in pet 28a in BL21(E.coli) but I am getting it as inclusion body. Please suggest me some way to solubilise the protein. I have tried expression at 16 with low IPTG..