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There's a possibility that your gel will warp if it heats up significantly during transfer, which can distort your protein bands. It really depends on how hot your transfer buffer gets. I usually transfer at about 300 mA for 90 minutes, and I never use an ice block because it never gets that hot in the chamber.
Back when we ran transfers at 1 A, I always ran those transfers in the cold room with an ice block though. Your markers coming out fine suggests to me that your gel didn't heat up excessively.
#146103 I forgot to put ice block in transfer buffer, does it matter?
Posted
on 30 November 2012 - 05:28 PM
