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vks

Member Since 28 Nov 2012
Offline Last Active Nov 28 2012 07:38 AM

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Active Posts 3
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Canada

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My research interests
Large scale Protein Purification, protein-protein interaction and peptide based drugs

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Website URL http://www.vitapharma.net

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In Topic: cellulase enzyme

28 November 2012 - 07:37 AM

If you can explain your problem in deatail, I may be able to help. My graduate thesis was on purification and characterization of cellulases from plant and microbial sources.
Best wishes,

vks

www.vitapharma.net
Experts of Protein Purification Products

In Topic: Characterization of enzyme in crude extract

28 November 2012 - 07:32 AM

Are you using full speactrum of broad range proteases in your lysis buffer? how much salt do you have in buffer? characterizing enzyme in very crude extract is of limited value, how about partial characterization using ammonium sulphate or acetone preocipitation?

best wishes,
vks

www.vitapharma.net
Protein Purification Reagents Experts