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  2. → Viewing Profile: Topics: Ensyeh

Ensyeh

Member Since 28 Nov 2012
Offline Last Active Mar 24 2013 05:13 AM
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Topics I've Started

The same CT with different Quantity, why?

06 March 2013 - 12:28 AM

Hello every one,
Im beginner in q-PCR .I had to repeat several experiments because they were unexpectable based on samples sites, but I've got some results and I have a question: the CT value is appx the same but the quantity is many different and I dont know why is happend this big difference and how to decide for the true ones? .
need your help

problem with plasmid digestion

16 January 2013 - 02:03 PM

Hi every one,
I have a question about digestion: I transformed my plasmid to Ecoli then purified it, then added teh restriction enzyme(sac I takara) for one restriction site and didnt get a good result (just supercoiled without restriction with lower weight than  linear plasmid), so I tried with sacI biolabs and got a sharp 3kb band uppr than former expriment but my band should be 4200 b appx.If my incubation time was low and my plasmid was digested incompletely why didnt see 2 band include ccc and linear form? and if it was complete why the band wasnt upper 4000?need help

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