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  2. → Viewing Profile: Posts: Ensyeh

Ensyeh

Member Since 28 Nov 2012
Offline Last Active Mar 24 2013 05:13 AM
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Posts I've Made

In Topic: The same CT with different Quantity, why?

06 March 2013 - 01:28 PM

Let me to ask my last question : how many times you are using the single standard dilution? and if you keep your standard in -70°C or -20?
thanks a lot.

In Topic: The same CT with different Quantity, why?

06 March 2013 - 12:39 PM

Why I didn't think to this great option myself? thank you much! (I was making the standard dilution each time). I should try it. thanks again.Posted Image

In Topic: The same CT with different Quantity, why?

06 March 2013 - 04:11 AM

Run1: slope-/3.1 /efficiency: 1o9 ,Run2: slope:-2.5 / efficiency:145 Run1: CTmean for 0.2 ng standard:12.3 Run2:CTmean for 0.2 ng standard:12.4. My supervisor believes that my standard is the problem, since although the amount of plasmid DNA are the same (based on photometry) but copy number of target gene are not the same.

In Topic: The same CT with different Quantity, why?

06 March 2013 - 02:20 AM

Thank you Veteran , I just want to measure the quantity of my gene of interest in environmental samples using the standard gene with the clear concentration(quantity). in the same quantity of my standard Ive got the same CT and my standard curve is fine . but for my sample it didnt happened and I have the same Ct mean with a big difference in the quantity Mean. (second experiment is showed by red).

Run1:Sample1:CT=31.44781/ CT Mean=31.40128/ CT Standard Deviation=0.065814/ Quantity=1.7*10^-7/ Quantity Mean=1.8*10^-7/QSD=8.5*10^-9/sample 1:CT=31.35474/CT Mean=31.40128/CT SD=0.065814/Quantity=1.9*10^-7/Quantity Mean=1.8*10^-7/QSD=8.5*10^-9/

Run2:sample1:CT= 31.87543/CT Mean=31.46424/CT SD=0.5815/Quantity=1.5*10^-8/Quantity Mean=2.3*10^-8/QSD=1.1*10^-8/Sample1:CT=31.05305/ CT Mean= 31.46424/CT SD=0.5815/Quantity=3*10^8/Quantity Mean= 2.3*10^-8/QSD=1.1*10^-8

In Topic: Negative Control Looks Like the Rest

05 March 2013 - 02:14 AM

Hello Member!
I dont know if you resolved your problem or no, but my idea is a contamination in your loading dye.

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