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Im currently transformed pGSA1131(binary vector) into agrobacterium competent cells. Since agrobacterium resistance to Rifampicin and pGSA1131 resistance to Chlorophenicol thus both antibiotic was used for colonies selective after transformation.There was a lot of colonies growth on petri dish with selective antibiotics (15ug/ml for rifampicin and 30ug/ml for chlorophenicol), BUt none of it given a positive result through PCR colony.
Does anyone faced similar problem as mine? Please kindly reply me..Im realy appreciate for your help.
Thanks in advance.
