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Hello, everyone,
I'm new here and interested in DNA methylation regulation. I use the EZ methylation kit to do treatment of bisufite, and then I stored my treated DNA at -80oC. Do someone have an idea that whether the proportion of methylation in bisulfited DNA will change with longterm conservation or frequent temperature changes (thawing for experiment)??? And have you tested it ? (I wonder the strand which contains uracil, it maybe unstable, so it's easy to break?)
Thans a lot !
Leon
