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bob1, on 22 November 2012 - 12:05 PM, said:
I think it should be still methylated, but I'm no expert...
Thank you !
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In Topic: Porportion methylation of bisulfite DNA changes with time?
21 November 2012 - 01:07 PM
bob1, on 21 November 2012 - 11:54 AM, said:
The methylations shouldn't be removed, but the integrity of the DNA and hence the dectection of the methylation by bisulphite PCR may be affected by freeze/thaw. Storage frozen at -80 should be enough to prevent degradation with time.
Thank you for your answer, so you mean that for the bisulfited DNA, the strands which contain uracils, and others which contain cytosines (methylated), the stability for these two strands are the same???
For example, I've a bisuflted DNA which is already known that it's 50% methylated by sequencing, and then I stored this bisulfited DNA (the same one which is sequenced) at -80 for about several weeks, and I re-send this bisulfited DNA to sequence, you think it'll still be 50% methylated???
Waiting for your answers...Thank you so much!
In Topic: How is bisulfite converted DNA unstable over time?
21 November 2012 - 04:46 AM
pcrman, on 25 April 2011 - 06:55 PM, said:
It should be stable for at least 6 months if properly stored at -20C.
Hello, I want to know 6 months, you mean that it will not change the proportion de methylation and unmethylation???
