Find content
Not Telling
Hello,
I've been having some trouble with my cell lysates in preparation for a western.
I collected my cells using RIPA buffer but after spinning them down to get rid of the nucleus, no pellet seems to show up.
We've had problems with our RIPA buffer before and finally got it fixed and this is the first time for me to use it ever since.
I went ahead and did the protein measurements using Bio-Rad assay but as expected, the measurements turned out to be very low or negative.
This is the second time in a row this has happened and I have no idea why the cells aren't getting lysed.
Any suggestions or help?
Thank you!
