I'm trying to concentrate a low number of LNCaP cells (50-200) in a large excess volume (1.5mL of PBS + 1%BSA+1mM EDTA), for culture in 96-well plates. I've tried spinning down the eppendorf containing the cells (740xg for 5 min), and pipetting the 1.5mL into the well plates directly, but cell recovery is very low (1-5%). I'm sure there is a smarter way to do this and would welcome advice, the only constraint being there is no way I can bring the intial volume down from 1.5mL, it's a limitation of the equipment I'm using. Thank you.
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