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Hey guys,
I'm having some problems with isolating RNA from anthers of Sorghum (Sorghum bicolor). I have tried a few protocols and no luck.
With the plant RNA reagent from invitrogen, a yellow/brown substance is in solution with the RNA (at the top of the solution after chloroform separation). It then precipitates with the RNA when isopropanol is added. I end up getting little to no RNA.
I have tried a protocol for high starch and the qiagen plant columns with no luck either. I dont know what is contaminating the aqueous phase. Any suggestions would be great!
Thanks
Ricky
