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Well..this is what I am looking for..Cancer cells do secrete growth factors all times..but are they all angiogenic stimulants? If am looking for angiogenic stimulating factors in the conditioned medium (ok..not just VEGF..but may be others too) shouldn't I grow the cancer cells in special environment that induces them to produce such factors?
I do use a recombinant VEGF directly, but am also interested in random factors, as in the conditioned medium.
Thank you..
