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hello
I have an insert is 200 bp.it is a pcr product. I did pcr purification and it is 138 ng/ul and 158 ng/ul. my vector is 6000 bp and 580 ng/ ul. I want to clonning but I am not . My restricition digestion enzymes NotI and PacI (FERMANTAS 10u/ul). I can not set of the amount of enzyme and vector and ınsert. how do I adjust the amount of reaction? fermantas is recommend
10X Buffer G
4-fold excess of NotI
4-fold excess of PacI
Incubate at 37 but ı can't understand.please help me.
