thanks for your information. ı did 20 unit enzyme for vector.because my teacher said to me not use for more enzymes.but ı changed the reaction conditions. 4 hour 37 centigrade degree and 12 hours 25 centigrade degree and 10 minute 65 centigrade degree. I use 2.5 ug vector for 20 unıt enzyme. Now I will do ligation but I don't know How ı adjust amount of insert vector. I tried to use ligation calculater system but ıt was said ... ng insert use. but I don't know after gel exraction How much vector and insert I have. I send the gel extraction picture.according to picture Could you help me ligation set up vector ınsert amount?
Find content
Not Telling
