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reeler

Member Since 13 Nov 2012
Offline Last Active Dec 13 2012 12:51 PM
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Topics I've Started

Quantify gDNA in buccal swab

13 November 2012 - 11:52 AM

Hi all,
I'm trying to perform PCRs direct on buccal swab material (no lysis, just buccal swab in water then vortex). I fear I'm getting very low DNA yield but what is the quickest way to check DNA quantity? Agarose gel would just be a smear,right?
OD260 would be meaningless with all cell debris RNA etc.?
Sorry if this has been asked before, I've been searching for half an hour and could find anything complete
Cheers!

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