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- It yields 5-10 ug of DNA from a dried specimen swab, instead of 200-500 ng.
- It improves detection sensitivity.
- It's nontoxic.
- Its lysate may be used for PCR without further DNA purification.
- Dried specimen swabs can be shipped and stored long-term at room temperature.
#146045 Quantify gDNA in buccal swab
Posted
on 29 November 2012 - 02:29 PM
DNA binds the cotton swab amazingly tight. Water can only elute ~5% of DNA from a dried specimen swab. We have developed an AquaGenomic methodthat can fully recover the DNA from dried specimen swabs. You can extract the DNA from the buccal swab with AquaGenomic and then quantify the DNA by conventional methods, from UV spec to real-time PCR.
#145178 Quantify gDNA in buccal swab
Posted
on 13 November 2012 - 11:59 AM
There isn't really any easy answer to this, your best bet would be to try some sort of fluorescence method, such as the Invitrogen QuantIT system, it might pick up RNA too, but that should typically be less than 5% of the total nucleic acid component of a cell.
