Find content
Not Telling
Hi everybody,
I want to test the effects of patients plasma on the prolifiration of allogenic PBMC activated by anti CD3/CD28 so i got some questions over my protocol:
do you think that i have to starve my cells from serum 24 hours before the test?
what is the concentration of plasma to be used (I'm thinking of 7,5% to 10% with RPMI)?
is there any need to evaluate the toxicity of the plasma on the allogenic PBMC (like viability test)?
I think about using a healthy donors serum like control?
Thx alot for your help
