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Hi PCRman,
I converted gDNA of a Rattus cell line by EZ DNA Methylation-Gold. I haven't achivied to amplify region of interest, yet. But I don't know whether primer pair work or converted DNA is adequate for PCR. Thus, I need a primer pair which conversion-specific and isn't contain CpG.
If there is a primer pair that working for bisulfite converted DNA amplification, could you send me sequence of primers and pcr conditions?
