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  2. → Viewing Profile: Posts: hj_chris

hj_chris

Member Since 11 Nov 2012
Offline Last Active Nov 20 2012 11:35 PM
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Posts I've Made

In Topic: Plasma effect on PBMC proliferation

12 November 2012 - 10:43 PM

I think you may need NC serum and FBS as your control.
I remember these is a paper from science, many years ago, used 20% serum. But you may need the titration.

In Topic: Mouse IgG detection - Blocking ?

12 November 2012 - 10:40 PM

you can try serial dilution. I used one sample as standard and did serial dilution from X5000 to X32000

In Topic: comment yield of BMDC (mDC) from 1 mouse

12 November 2012 - 12:55 AM

Anyone know?
I can only get 6 million BMDC from 1 mouse, and only 1 million BMDC after transfection and 5 days culture. Is that too small population?
Thanks for your help!!!

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