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Yeah, I would plot the growth and make sure both are in log phase then take samples, and making sure that both give the same OD. In the end you are examining the RNA, so technically the growth of the bacteria wouldn't matter too much so long as you put equal amounts of RNA into the cDNA reaction and so into the qPCR.
#145002 How to Compare CT values between treated and untreated bacteria with uneven grow
Posted
on 10 November 2012 - 01:03 PM
