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Mettiu

Member Since 08 Nov 2012
Offline Last Active Mar 22 2013 01:51 AM

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Neurobiology

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Curtis
15 Jan 2013 - 18:08

Topics I've Started

Problems with extraction of RNA from primary culture

15 January 2013 - 09:00 AM

Hi there,

I am working with mixed glial/neuronal primary culture (P1&P2) from cortex. I am having some problems with the extraction of RNA.
From 2 X 10^6 cells per well I can't get more than 70ng/ul. I have used kits like Qiagen, Roche, and TRIZOL.

Does anybody have any suggestion?

Thanks

omega-3s diets

15 January 2013 - 03:06 AM

Hi there,

I need to figure out a way to mash fatty acids (oils) in a normal chow for rats.

Thanks,

Question - Corticosterone, cell culture

08 November 2012 - 08:11 AM

Is there anybody that have experience with stress/corticosterone induced in neuronal cell cultures? I would like to have information about how to dissolve the corticosterone in the cell culture media. Thanks