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  2. → Viewing Profile: Topics: Chikopiko

Chikopiko

Member Since 07 Nov 2012
Offline Last Active Private
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Topics I've Started

96 well migration chambers

03 December 2012 - 12:44 PM

Hello,
Does anyone have any recommendation for 96 well inserts for migration assay?

Thank you!!!

96 well migration assay

29 November 2012 - 12:45 PM

Hello,
Can anyone recommend good 96 well migration assay plates? I used the millipore plates and they leaked from the upper chamber to the bottom chamber.  Any suggestions??

Peprotech IP-10 ELISA

16 November 2012 - 09:24 AM

Hello,
I am using the Peprotech kit for IP-10 Elisa. I was using the standards provided by the kit and did serial dilutions, the last point of 0 pg/ml was used as diluent alone. However, when I was measuring my results I realized that some of the tested cells gave me lower O.D values than the O.D of the 0 pg/ml Standard. Thus when I calculated the IP-10 values in my experiment some of the tested wells had a negative value in terms of pg/ml. Has anyone experienced suce a thing? Is there any way to sove this issue? Also It took a while (about 30 min) until the color developed to give O.D values of 0.9 for the 1000 pg/ml standard.

Thank you!!!!

Lysis buffer

07 November 2012 - 11:46 AM

Hello,
I have found several protocols for adherent cell lysis buffer (NP-40). Some have 10mM,20mM or 50mM Tris HCl. Why is there a diference? Also, it was suggested that RIPA buffer is not compatible with Bio-Rad bradford assay reagent, however in the guidelines of the reagent it is mentioned that up to 0.1% SDS is compatible. Any recommendations for good lysis buffer protocols for both membrane and cytosolic protein extraction?

Thanks!!

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