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Hi!
I ran a classical first and second round PCR reaction. I got a very weak band of my desired product from the 2nd round PCR. After many trials, I dicided to run a third PCR round from the product and conditions of the 2nd round; I finally got a big band of the desired product. But after purification with ZYMOGEN, I still experience a negative value of the ratio 260/230 indicating a contamination. So is the problem with the 3th PCR round or the cleaning up prossess? My product is about 1Kb.
Thanks
