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azadeh12345

Member Since 06 Nov 2012
Offline Last Active Dec 14 2012 11:40 AM
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Topics I've Started

how can I remove the pigments during DNA extraction?

14 December 2012 - 06:32 AM

hi
I want to extract the DNA of a biotrophic fungi from the host tissue, this fungus causes the tissue turn to red.
After DNA extraction I have red pigments in my total DNA. also I have low PCR product, which I think is due to some inhibitors (probably the pigmets). how can I remove them?
Thank you.

how to amplify a fungus which is in the host tissue

07 November 2012 - 12:12 AM

hi
how can I amplify a fungus ITS region which is in the host tissue? my fungus is a biotroph and cannot be cultured, and I have to amplyfy it from the host tissue.
I have extracted the DNA of my infected leaves in which I have both the DNA of the fungus and plant,  and I have used primer ITS1 and ITS4, I have got two bands , I extracted the bands from gel, and sequenced them. one was the ITS of plant and the other was the ITS of my fungus.
but is there any other way to amplify only the fungal band?

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