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bob1

Member Since 21 Jul 2004
Offline Last Active Yesterday, 05:46 PM

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Community Stats

Group Global Moderators
Active Posts 4,340
Profile Views 31,343
Member Title Thelymitra pulchella
Age Age Unknown
Birthday Birthday Unknown
Gender
Male

About me

Real Name
Rhodri Harfoot
Lab or Group
Virus Research Unit
Institution
University of Otago
My research interests
Currently cell biology and virology. Interested in technique development and biochemistry.

Contact Information

Website URL http://

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bob1 → ACN

Hi, Why do you remove the agar? If you are trying to get the virus for recovery you can just stab through the agar with a pipette tip.

Jul 16 2011 09:33 PM

Hello, it seems like you know a thing or two about cell culture.
I have a question about a plaque assay I am trying to do. I know the virus (MCMV) I am using works; however, the cells (3T3, grown in DMEM+10%FCS+PSG+NaHCO3) keep coming off the plate (I believe when I remove the agar). Do you have any suggestions?

Jul 15 2011 11:46 AM

semperfi → bob1

Thank you for your reply
Many thanks

Jul 09 2011 11:04 AM

bob1

Hi Swizla21, I haven't worked on THP-1 cells before, although a lot of the principles of general cell culture will apply. All I know of them is that they are a cell line that can differentiate into macrophages on stimulation, which is usually done with LPS (lipopolysaccharide).

Apr 10 2011 04:12 PM

swizla21 → bob1

Hi Bob1, are you still working with THP 1 cells? Im just starting to fiddle with them and have a few questions.

Apr 09 2011 04:48 PM

bob1 → Meijles

Hi,
Here's a paper discussing that topic:
Traffic. 2010 Jun;11(6):732-42. Epub 2010 Feb 27.
Modifications to the C-terminus of Arf1 alter cell functions and protein interactions.
Jian X, Cavenagh M, Gruschus JM, Randazzo PA, Kahn RA.
Cheers,
Bob

Feb 02 2011 03:44 PM

Meijles → bob1

Many thanks for your words of wisdom regarding protein tags. Do you happen to know of any examples where the addition of a tag ultimately altered protein function through mis-folding?

Feb 02 2011 12:28 AM