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- Active Posts 4,344
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- Member Title Thelymitra pulchella
- Age Age Unknown
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Gender
Male
About me
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Real Name
Rhodri Harfoot
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Lab or Group
Virus Research Unit
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Institution
University of Otago
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My research interests
Currently cell biology and virology. Interested in technique development and biochemistry.
Posts I've Made
In Topic: Role of sodium acetate in DNA extraction/precipitation
Today, 01:02 PM
The role is to increase the number of ions in solution to a point where the DNA can be precipitated by the addition of an alcohol primarily. In some extractions such as plasmid preps, it is used to neutralize the alkaline component of the lysis (step 2 NaOH and SDS) and precipitate the proteins and genomic DNA from this step, again through ionic strength.
In Topic: Stain in electrophoresis is going from + to -
Today, 12:44 PM
You should be able to add some stain to the running buffer.
In Topic: Citrate synthase standard for CS assay
Yesterday, 05:23 PM
Units for enzymes are usually defined by the amount of substrate per time. for many the unit of time is an hour - so perhaps this is your problem?
In Topic: colony formation assay
20 May 2013 - 12:36 AM
You can change the medium as this would be similar to giving repeated doses as a therapy, though it depends if you want this to be the case or not.
In Topic: What housekeeper to use with HEK cells?
18 May 2013 - 05:44 PM
Potentially, you should test several housekeepers to find the ones (you should be using 2-3) that are most stable.
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