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Thidarat Wi

Member Since 02 Nov 2012
Offline Last Active Nov 19 2012 12:54 AM
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Topics I've Started

Help with media filtration

02 November 2012 - 08:36 AM

Hi

I'm new in cell culture lab. Now I start to prepare media and other reagents. My cells is cultured by using mixed MEM and F-12 (50:50). I use a filter that can be used only once for 3 liter of media. But I don't want to filter a lot of media. If I filter 3 liter of each media, I will get 6 liter of media for cell culture!!! It is too much for doing my lab and  I also worry about the contamination after it is filtered..

So I have a question that can I use the same filter with different media?

Thanks a lot.

Thidarat Wi

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