I am a Phd student, my project based on study DNA methylation in small cell no. I have found this paper which stated primers, they used similar sample type. (table in page 534) I want to order the primers, but i have a Q when i run my MSP, which primer i used in first run and which i will use in my 2nd run? I am really confused!
paper title:Characterization of a New NIH-Registered Variant Human Embryonic Stem Cell Line, BG01V: A Tool for Human Embryonic Stem Cell Research
sorry i can't attach the paper! but i copy past the primer that i interested in...
H19 promoter region L-Unmethylated primer L-Methylated primer
H19 promoter region R-Unmethylated primer R-Methylated primer
I hope find the answer !
LailaMember Since 02 Nov 2012
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