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Laila

Member Since 02 Nov 2012
Offline Last Active Dec 07 2012 06:51 AM

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Epigenetics

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casandra
15 Nov 2012 - 07:05
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07 Nov 2012 - 07:48

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Q regarding primer mix for MSP!!

02 November 2012 - 08:23 AM

Hi,

I am a Phd student, my project based on study DNA methylation in small cell no. I have found this paper which stated primers, they used similar sample type. (table in page 534) I want to order the primers, but i have a Q when i run my MSP, which primer i used in first run and which i will use in my 2nd run? I am really confused!

paper title:Characterization of a New NIH-Registered Variant Human Embryonic Stem Cell Line, BG01V: A Tool for Human Embryonic Stem Cell Research
sorry i can't attach the paper! but i copy past the primer that i interested in...

H19 promoter region                    L-Unmethylated primer                                                     L-Methylated primer
                                                  5􏰅-TTTTAGGAATGTGAGGTTTGAGTT-3􏰅                 5􏰅-TTTAGGAACGTGAGGTTTGAGTC-3􏰅

H19 promoter region                 R-Unmethylated primer                                                            R-Methylated primer
                                         5􏰅-CCTACTACTCCCTACCTACCAACAC-3􏰅              5􏰅-CCTACTACTCCCTACCTACCAACG-3􏰅

I hope find the answer !

regards,

Laila