wenchien

Hi john,
thank you for your reply,
I try bothe silver stain and SYPRO RUBY staining to develop the page.
My idea is to see 2:1 ratio on the PAGE
but Unlike the intensity of heavy chain, the intensity band of light chain is very weak in SYPRO RUBY and barely can not see when developing with silver.
I was wondering what cause these phonemonem
Another problem is the band of light chain shows smere instead of "one band" shape of heavy chain
At the begining, we thought it might cause by degredation and we took the new rabbit IgG but get same result.
Is there any other possibilty to cause these?


You mentioned about the mAb and pAb and where its epitopes are will affetc the binding of heavy and light chains.
what kind of result will I get when I use mAb and what in pAb?

Kindly appteciate your help
wen