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  2. → Viewing Profile: Topics: vivlee

vivlee

Member Since 30 Oct 2012
Offline Last Active Nov 15 2012 10:19 PM
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Topics I've Started

TFE in inducing alpha-helix formation in proteins

30 October 2012 - 05:24 PM

Hi all,

I have been doing TFE titraion on polypeptides at 4 degrees celcius using CD, and noticed its varying effects on different polypeptides. It was effective in inducing alpha-helical structure formation in one polypeptide but not another. One of the peptide I tested was a 16mer and the other is a 32mer, the 16mer is a part of the 32mer. Does the length of the peptide influence the effect of TFE, since TFE showed its effect on the 32mer but not the 16mer (even at 80%TFE)? Any other possible reason for the difference in TFE effects on the two mers I tested?

Thanks!

How to avoid gel breakage after electrophoresis

30 October 2012 - 05:14 PM

Hi all!
I have been running 8% SDS-gel (acrylamide) and the gel often breaks easily when I try to remove it from the glass plate after electrophoresis into a container for staining. I usually keep the gel wet during transferring into the container by letting deionized water running on the plate when I am trying to life the gel, though sometimes succussful, minor breakages still occur at times. Breakages also occurs at time when I need to take the gel out of the container for digital imaging after the staining.
Any SDS-PAGE experts out there that can help me minimize the chance of obtaining a broken gel? Thanks!

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