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madelingirly, on 30 October 2012 - 02:39 PM, said:
Dear
thebromar,
Once I got cells from ATCC, and the protocol was just like that, start culturing without removing of DMSO, and I did this and it was fine.
Then I searched online and I read that cells can withsatnd DMSO for 12-24 hrs, so I tried with another type of cells, I took my cells from liquid nitrogen, cultured them without removal of DMSO, just putting it in medium.
and my cells were fine too, my cells did not die.
I guess this is not a common step to do, but what I mean that sometimes there are types of cells very sensitive like those I got from ATCC and better to be culture without removal of DMSO for 24 hrs before u can remove ur DMSO
thebromar,
Once I got cells from ATCC, and the protocol was just like that, start culturing without removing of DMSO, and I did this and it was fine.
Then I searched online and I read that cells can withsatnd DMSO for 12-24 hrs, so I tried with another type of cells, I took my cells from liquid nitrogen, cultured them without removal of DMSO, just putting it in medium.
and my cells were fine too, my cells did not die.
I guess this is not a common step to do, but what I mean that sometimes there are types of cells very sensitive like those I got from ATCC and better to be culture without removal of DMSO for 24 hrs before u can remove ur DMSO
Perfect. So actually, my cells were in DMSO for less than 24 hours, probably between 20-22. So, should be okay ,despite staying in 4h longer than I wanted. Thanks!
