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iratxe

Member Since 29 Oct 2012
Offline Last Active Oct 29 2012 09:52 AM
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Topics I've Started

separate plant protoplast from bacteria‏ prior DNA extraction

29 October 2012 - 07:33 AM

Hi all

My work is focused on microbes-plant interaction using metagenomics. I have extracted DNA from Leaves and fruits and around 95% of the sequences i obtained were from the choroplast and mitochondria from the plant, and actually i am interested in the bacteria growing in this samples.

does someone know a protocol to separate the plant protoplast from the bacteria to use it before performing the DNA extraction?

Thank you so much for your help
Iratxe

by the way...congrats for the forum, it is really interesting!

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