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Morteza Moghadam

Member Since 29 Oct 2012
Offline Last Active Oct 29 2012 04:49 AM

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Phil Geis
29 Oct 2012 - 12:27

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How long Kanamycin can last in broth medium?

29 October 2012 - 04:52 AM

I made my Pseudomonas bacteria resistant to kanamycin (kan), However, when I grew the wild type and the recombinant one in LB broth medium containing kanamycin for 48 hours, as expected

, nothing grew on my negative control medium (Kan containing medium inoculated with wild type strains) while recombinant bacteria could grew happily. However, on the 3rd day, my negative control media became turbid Posted Image

!!
Anyone has any explanations?
Is it possible that kanamycin had lost its efficiency after 3 days? or my wild type strains could manage to become Kan resistant? Posted Image

Thank you so much for your replies...