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Dear all
I have the problem of immunoprecipitation. Please help me solve this problem.
I overexpressed the HA-tag expression vector in cells，and i can detect the exogenous HA tag protein by using western blotting.
But when I perform immunoprecipitation, I used HA tag antibody to pull down the protein complex of my HA-tag protein. I cant detect the HA-tag protein in the pulldown protein complex by using western blotting. My HA-tag protein has many zinc finger, i think whether the HA tag is embed in the protein structure, so HA tag antibody cant pull down this protein.
Please help me disolve this problem.
Thanks very much.
