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That is what I was afraid of. I knew the first step when I added the Lysis/Binding Solution would lyse the cells like Trizol does, but I also figured it had something bind to the miRNA given its name, which would not allow it to elute through the filters they provided.
I was thinking that I just go ahead and start with adding the Lysis/Binding Solution and continue the protocol from there, since the cells are already lysed I am hoping there would be no adverse effects and that the binding part will just do its job, I just hope that Trizol does nothing to mess up the reaction.
I will definitely call the companies up to see if they may have a work around. I was kind of hoping that someone here ran into the same problem, but I guess not.
Thank you bob1.
