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Cell count and seed, plz help...
27 October 2012 - 01:56 PM
Hello, I have a doubt regarding the seeding specific number of cells after counting with Hemocytometer. If for example we have 10ml of cell suspension, we take few ul of the suspension and count with Hemocytometer and the count is 300,000 (it means this 300,000 is in 1ml). If we want to seed 200,000 cells, what to do? Do we need to calculate the number of cells in 10 ml first which will be 3000,0000 cells and take 0.066ml OR we should consider the number of cells from Hemocytometer (300,000/ml) and take 0.66ml ? It would be great if someone can help me with this with some kind of explanation. Thank you very much
