Thanks for the Reply,
I had desided to keep my kit at 4°C, Room Temperature and at 37°C.
For Proteinase K & Lysozyme: Though we know that these enzyme will be in active state to some extent at RT and 37°C, is it necessary to keep these Enzyme at such temperature? can I put them with remaining buffers?
For Samples: What kind of sample should I use? My kit can be use to isolate DNA from both, Gram positive as well as Gram negative cultures. Should I use fresh cultures of E.Coli & B. subtillis?
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