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ipman_81

Member Since 24 Oct 2012
Offline Last Active May 17 2013 02:43 PM
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Posts I've Made

In Topic: Questions / Help with Fusion PCR

26 November 2012 - 03:26 PM

Finally got it to work like everyone else's!! Ok so it seems as though the mid-piece had some issues. I confirmed the primer sequence for amplifying the midpiece and did an experiment to test between what I had been using this whole time and a newly amplified midpiece fragment. The new ones came out almost flawless! =)

So I think the lesson is that even if people in the lab have done it a thousand times, make sure you understand the method thoroughly and double check everything that is handed down to you.

In Topic: Questions / Help with Fusion PCR

16 November 2012 - 10:56 AM

Ok so now I'm really sad. The Fusion PCR reaction is working very efficiently in other peoples hands in the lab. What could I be doing wrong that everyone is doing right?

In Topic: Questions / Help with Fusion PCR

25 October 2012 - 05:02 PM

Well the method of not adding primers until a few cycles didn't work. I thought that was a really good idea though. Thank you OA17.

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