when the rate of a specific mutation is more than 1% IN THE POPULATION,then it is calld polymorphysm (or single nucleotide polymorphysm) but when it's prevalence in the population is less than 1%,it;s called a mutation.
BSP is performed using bisulfite-modified DNA as the template using primers that selectively amplify bisulfite-modified DNA and contain no CpG sites so that both methylated and unmethylated DNA are amplified. The resulting PCR product can then be used in the following three ways:
(1) direct sequencing to examine strand-specific methylation for all population of molecules in the DNA sample;
(2) cloning and sequencing to study the methylation status of individual molecules;
(3) digestion with restriction enzymes to examine methylation of particular CpG sites recognized by the enzymes.
BSP is performed using bisulfite-modified DNA as the template using primers that selectively amplify bisulfite-modified DNA and contain no CpG sites so that both methylated and unmethylated DNA are amplified. The resulting PCR product can then be used in the following three ways:
(1) direct sequencing to examine strand-specific methylation for all population of molecules in the DNA sample;
(2) cloning and sequencing to study the methylation status of individual molecules;
(3) digestion with restriction enzymes to examine methylation of particular CpG sites recognized by the enzymes.
Find content
Not Telling
