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Hi,
I use to quantify microRNAs by EXIQON kit (mircuryLNA), that, as far as I see, is one of the best. You can use other endogenous controls such as 5S, RNU5G, RNU1A1, that are small RNA. I'm sorry but I don't have sequences since I use to buy them directly from EXIQON, but I'm sure you can find them somewhere. However, I would sugest to give a look on EXIQON website, even if you are not using their kit, there are good sugestion about miRNA quantification.
Good luck!
alessandra
#143604 miRNA qPCR normalization/control gene sequence!!!!
Posted
on 18 October 2012 - 02:17 AM
