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Try it and see -my personal experience (10ish years) is that it doesn't work between gels, no matter how good you are at westerns, even with housekeepers, unless you can discount the variations in transfers, exposures, antibody steps, washes, etc.
#125111 Western blots are 'semi' quantitative?
Posted
on 08 December 2011 - 06:42 PM
#124908 Western blots are 'semi' quantitative?
Posted
on 05 December 2011 - 07:11 PM
You can try, but you will find the results are very variable. You need to be able to load the same amount of total protein into each lane, transfer it all evenly, and then get nice exposures that are not over or under exposed consistently for each membrane that you run. I have found it to be next to impossible to get exactly the same (or even similar) results for the same lysates run at the same time on different gels, transferred at the same time in the same blot apparatus, and probed in the same solutions with all conditions identical...
