Hi there, I am just wondering of why were you normalizing for lipid droplet concentration? Wouldn't you need to normalize for protein content instead? I think your idea that the lipid droplet might affect the result is a possibility. The SDS Page is a highly charged system so my guess is that having something hydrophobic could lead to your samples to float/pull away the protein that is supposed to run along with the system. To be sure, I am just wondering if you could perhaps defat your samples so that you will only be running the protein fraction that was previously associated with the lipid fraction?
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